In Silico Evaluation of Lawsonia intracellularis Genes Orthologous to Genes Associated with Pathogenesis in Other Intracellular Bacteria

Author:

Suarez-Duarte Mirtha E.1,Santos Renato L.1ORCID,Pereira Carlos E. R.2,Resende Talita P.3,Araujo Matheus D.1ORCID,Correia Paula A.1,Barbosa Jessica C. R.1,Laub Ricardo P.1,Rodrigues Diego L. N.4ORCID,Aburjaile Flavia F.4ORCID,Guedes Roberto M. C.1ORCID

Affiliation:

1. Department of Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil

2. Department of Veterinary, Universidade Federal de Viçosa, Viçosa 36570-900, Minas Gerais, Brazil

3. Department of Animal Science, College of Food, Agriculture and Environmental Sciences, Ohio State University, Columbus, OH 43210, USA

4. Department of Preventive Veterinary Medicine, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil

Abstract

Proliferative enteropathy is an enteric disease caused by the bacterium Lawsonia intracellularis, which affects several species of domestic and wild animals. The mechanisms underlying the mechanisms employed by L. intracellularis to cause host cell proliferation are poorly understood, mostly because this bacterium is extremely difficult to isolate and propagate in vitro. Comparative genomics methods for searching for genes orthologous to genes known to be associated with pathogenesis allow identification of genes potentially involved in pathogenesis by the pathogen of interest. The goal of this study was to carry out in silico research on L. intracellularis genes orthologous to genes required for intracellular invasion and survival present in other pathogenic bacteria, particularly Brucella abortus, B. melitensis, B. suis, Listeria monocytogenes, Mycobacterium tuberculosis, Mycobacterium avium subspecies paratuberculosis, Salmonella enterica, Yersinia pestis, Y. enterocolitica, and Y. pseudotuberculosis. A total of 127 genes associated with invasion and intracellular survival from five known intracellular bacteria were mapped against the predicted proteomes of all L. intracellularis strains publicly available on GenBank, using the OrthoFinder program. A total of 45 L. intracellularis genes were orthologous to genes associated with pathogenesis of other intracellular bacteria. Genes putatively associated with signal the transduction of chemotaxis and cell motility were identified. Genes related to DNA binding and repair were also identified, with some of them supporting a possible association of bacteria with macrophages or inducing pro-inflammatory responses. The homology-based identification of these genes suggests their potential involvement in the virulence and pathogenicity of L. intracellularis, opening avenues for future research and insights into the molecular mechanisms of Lawsonia-elicited proliferative enteropathy.

Funder

FAPEMIG

CAPES

CNPq

Capes Foundation, Ministry of Education, Brazil

Publisher

MDPI AG

Reference43 articles.

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5. Metagenomic sequencing of clinical samples reveals a single widespread clone of Lawsonia intracellularis responsible for porcine proliferative enteropathy;Bengtsson;Microb. Genom.,2020

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