Clinical Characteristics, Antimicrobial Resistance, Virulence Genes and Multi-Locus Sequence Typing of Non-Typhoidal Salmonella Serovar Typhimurium and Enteritidis Strains Isolated from Patients in Chiang Mai, Thailand
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Published:2023-09-28
Issue:10
Volume:11
Page:2425
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ISSN:2076-2607
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Container-title:Microorganisms
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language:en
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Short-container-title:Microorganisms
Author:
Buddhasiri Songphon1ORCID, Sukjoi Chutikarn2, Tantibhadrasapa Arishabhas2, Mongkolkarvin Panupon2, Boonpan Pattarapon2, Pattanadecha Thanakorn2, Onton Nattamon2, Laisiriroengrai Touch2, Coratat Sunatcha2, Khantawa Banyong3, Tepaamorndech Surapun4, Duangsonk Kwanjit2, Thiennimitr Parameth256ORCID
Affiliation:
1. Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand 2. Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand 3. Diagnostic Laboratory, Maharaj Nakorn Chiang Mai Hospital, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand 4. Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand 5. Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50100, Thailand 6. Center of Multidisciplinary Technology for Advanced Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
Abstract
Non-typhoidal salmonellosis (NTS) caused by ingesting Salmonella enterica contaminated food or drink remains a major bacterial foodborne disease. Clinical outcomes of NTS range from self-limited gastroenteritis to life-threatening invasive NTS (iNTS). In this study, we isolated Salmonella spp. from the stool and blood of patients hospitalized at Maharaj Nakorn Chiang Mai Hospital, Chiang Mai, Thailand, between 2016–2021 (a total of 395 cases). Then, serovar Typhimurium and Enteritidis were identified and further characterized by multiplex PCR, and multi-locus sequence typing. Our data show that multidrug resistance (MDR) sequence type 34 (ST34) and ST11 are the predominant sequence types for serovars Typhimurium and Enteritidis, respectively. Most S. Typhimurium ST34 lacks spvB, and most S. Enteritidis ST11 harbor sseI, sodCI, rpoS and spvB genes. NTS can be found in a wide range of ages, and anemia could be a significant factor for S. Typhimurium infection (86.3%). Both S. Typhimurium (6.7%) and S. Enteritidis (25.0%) can cause iNTS in immunocompromised patients. S. Typhimurium conferred MDR phenotype higher than S. Enteritidis with multiple antibiotic resistance indexes of 0.22 and 0.04, respectively. Here, we characterized the important S. Typhimurium, S. Enteritidis, and human clinical factors of NTS within the region.
Funder
Faculty of Medicine, Chiang Mai University, Thailand Thailand Health Systems Research Institute Thailand Research Fund Chiang Mai University Research Fund
Subject
Virology,Microbiology (medical),Microbiology
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