Affiliation:
1. Research Laboratory “Agrobioengineering”, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia
Abstract
Due to their capacity to produce antimicrobial peptides that can prevent the growth of diseases, many Bacillus spp. are beneficial to plants. In this study, we looked into the antagonistic activity of the B. pumilus 3-19 strain and its derivatives following targeted genome editing. Two peptide genes with antibacterial action, bacilysin (bac) and bacteriocin (bact), and the sigF gene, which encodes the sigma factor of sporulation, were specifically inactivated using the CRISPR-Cas9 system in the genome of B. pumilus 3-19. Antibacterial activity against B. cereus and Pantoea brenneri decreased as a result of the inactivation of target genes in the B. pumilus 3-19 genome, with a noticeable effect against bacilysin. The growth dynamics of the culture changed when the bac, bact, and sigF genes were inactivated, and the altered strains had less proteolytic activity. An asporogenic mutant of B. pumilus 3-19 was obtained by inactivating the sigF gene. It has been proven that bacilysin plays a unique part in the development of B. pumilus 3-19’s antagonistic action against soil microorganisms.
Funder
Russian Science Foundation
Subject
Virology,Microbiology (medical),Microbiology
Reference38 articles.
1. Impact of exogenous silicon addition on chromium uptake, growth, mineral elements, oxidative stress, antioxidant capacity, and leaf and root structures in rice seedlings exposed to hexavalent chromium;Tripathi;Acta Physiol. Plant.,2012
2. Niazi, A., Manzoor, S., Asari, S., Bejai, S., Meijer, J., and Bongcam-Rudloff, E. (2014). Genome analysis of Bacillus amyloliquefaciens subsp. plantarum UCMB5113: A rhizobacterium that improves plant growth and stress management. PLoS ONE, 9.
3. Portraying mechanics of plant growth promoting rhizobacteria (PGPR): A review;Goswami;Cogent Food Agric.,2016
4. Bacillus: A biological tool for crop improvement through bio-molecular changes in adverse environments;Radhakrishnan;Front. Physiol.,2017
5. Potential of Bacillus pumilus to directly promote plant growth;Dobrzynski;Front. Microbiol.,2022
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献