Responses of Maize (Zea mays L.) Roots to Nitrogen Heterogeneity and Intraspecific Competition: Evidence from a Pot Experiment Using the ‘Root Splitting’ Approach

Abstract

A heterogeneous distribution of nutrients in the soil and the root interactions of crops coexist in agroecosystems. A ‘root splitting’ approach, i.e., splitting the root system into different nutrient compartments, was used to study nutrient heterogeneity distributions and root interactions. We evaluated root foraging behavior, individual growth, and root development mechanisms in response to soil nitrogen heterogeneity, and intraspecific competition in maize. A heterogeneous distribution of nitrogen increased the foraging precision of roots at flowering and mature stages (Foraging precision > 1), and significantly increased root biomass and surface area on the intraspecific competition and no competition on the high nitrogen side. The heterogeneous distribution had no effect on yield, total root biomass, specific root length, total surface area, or average diameter. Intraspecific competition increased inter-crop competition (RII < 0) and reduced total root biomass, total plant biomass, and the root/shoot ratio. These results inform root trait development studies and provide valid evidence for optimizing and managing fertilizer application in agroecosystems, helping to maximize crop yield and nutrient use efficiency.