Exploring the Molecular Landscape of Nitrogen Use Efficiency in Potato (Solanum tuberosum L.) under Low Nitrogen Stress: A Transcriptomic and Metabolomic Approach

Abstract

Enhancing crop nitrogen use efficiency (NUE) in agricultural sciences is a pivotal challenge, particularly for high-demand crops like potatoes (Solanum tuberosum L.), the world’s third most significant food crop. This study delves into the molecular responses of potatoes to low nitrogen (LN) stress, employing an integrative approach that combines transcriptomics and metabolomics to compare two cultivars with divergent NUE traits: XS6, known for its high NUE, and NS7, characterized by lower NUE. Our research unveils that XS6 exhibits higher chlorophyll and N content, increased tuber yield, and elevated N assimilation capacity under LN stress conditions compared to NS7. Through transcriptome analysis, we identified critical genes involved in C and N metabolism that had higher expression in XS6. A significant discovery was the high-affinity nitrate transporter 2.7 gene, which showed elevated expression in XS6, suggesting its key role in enhancing NUE. Metabolomics analysis further complemented these findings, revealing a sophisticated alteration of 1252 metabolites under LN stress, highlighting the dynamic interplay between carbon and N metabolism in coping with N scarcity. The integration of transcriptomic and metabolomic data underscored the crucial role of trehalose in mitigating N deficiency and enhancing NUE. This study provides novel insights into the molecular mechanisms governing NUE in potatoes, offering valuable perspectives for molecular breeding to enhance NUE in potatoes and potentially other crops.