Identification and Genetic Diversity of Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) in Egypt

Abstract

Fall armyworm, Spodoptera frugiperda, is a serious agricultural pest native to tropical and subtropical regions of the Western Hemisphere and has invaded Africa and further spread into most countries of Asia within two years. In Egypt, researchers have conducted thorough investigations into the behavior of the fall armyworm and various methods to manage its impact. This study aims to further our understanding of the genetic diversity of fall armyworm populations in Egypt. By collecting specimens from five provinces across the country, we sought to analyze their genetic makeup. Through examination of partial sequences of the mitochondrial cytochrome oxidase subunit I (COI), we identified three distinct haplotypes. Phylogenetic analysis suggests that the primary sources of Spodoptera frugiperda in Egypt likely stem from both Asian and African origins. Employing the PCR-RFLP technique on the complete COI sequence, we were able to discern genotype patterns within the fall armyworm population. Our findings indicate the presence of two distinct strains—the Corn and Rice strains—within Egypt. This research contributes essential insights into the genetic diversity of insects in Egypt, providing valuable knowledge that can inform more effective strategies for managing this agricultural pest.