Development of a Novel Peptide with Antimicrobial and Mineralising Properties for Caries Management
-
Published:2023-10-31
Issue:11
Volume:15
Page:2560
-
ISSN:1999-4923
-
Container-title:Pharmaceutics
-
language:en
-
Short-container-title:Pharmaceutics
Author:
Zhang Olivia Lili1, Niu John Yun1ORCID, Yu Ollie Yiru1ORCID, Mei May Lei12ORCID, Jakubovics Nicholas Stephen13ORCID, Chu Chun Hung1ORCID
Affiliation:
1. Faculty of Dentistry, The University of Hong Kong, Hong Kong 999077, China 2. Faculty of Dentistry, The University of Otago, Dunedin 9054, New Zealand 3. School of Dental Sciences, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4BW, UK
Abstract
The purpose of the study is to develop a novel peptide for caries management. Gallic-Acid-Polyphemusin-I (GAPI) was synthesised by grafting Polyphemusin I (PI) and gallic acid (GA). Biocompatibility was evaluated using a Cell Counting Kit-8 Assay. Antimicrobial properties were assessed using minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC). The bacterial and fungal morphology after GAPI treatment was investigated using transmission electron microscopy (TEM). The architecture of a consortium biofilm consisting of Streptococcus mutans, Lacticaseibacillus casei and Candida albicans was evaluated using scanning electron microscopy (SEM) and confocal laser scanning microscopy. The growth kinetics of the biofilm was examined using a propidium monoazide–quantitative polymerase chain reaction. The surface and calcium-to-phosphorus molar ratio of GAPI-treated enamel after pH cycling were examined with SEM and energy-dispersive X-ray spectroscopy. Enamel crystal characteristics were analysed using X-ray diffraction. Lesion depths representing the enamel’s mineral loss were assessed using micro-computed tomography. The MIC of GAPI against S. mutans, L. casei and C. albicans were 40 μM, 40 μM and 20 μM, respectively. GAPI destroyed the biofilm’s three-dimensional structure and inhibited the growth of the biofilm. SEM showed that enamel treated with GAPI had a relatively smooth surface compared to that treated with water. The calcium-to-phosphorus molar ratio of enamel treated with GAPI was higher than that of the control. The lesion depths and mineral loss of the GAPI-treated enamel were less than the control. The crystallinity of the GAPI-treated enamel was higher than the control. This study developed a biocompatible, mineralising and antimicrobial peptide GAPI, which may have potential as an anti-caries agent.
Subject
Pharmaceutical Science
Reference41 articles.
1. Antimicrobial peptides (AMPs): Ancient compounds that represent novel weapons in the fight against bacteria;Ageitos;Biochem. Pharmacol.,2017 2. Zhang, O.L., Niu, J.Y., Yin, I.X., Yu, O.Y., Mei, M.L., and Chu, C.H. (2023). Bioactive Materials for Caries Management: A Literature Review. Dent. J., 11. 3. Antimicrobial peptides: Mechanism of action, activity and clinical potential;Zhang;Mil. Med. Res.,2021 4. Zhang, O.L., Niu, J.Y., Yu, O.Y., Mei, M.L., Jakubovics, N.S., and Chu, C.H. (2023). Peptide Designs for Use in Caries Management: A Systematic Review. Int. J. Mol. Sci., 24. 5. Zhang, O.L., Niu, J.Y., Yin, I.X., Yu, O.Y., Mei, M.L., and Chu, C.H. (2022). Growing Global Research Interest in Antimicrobial Peptides for Caries Management: A Bibliometric Analysis. J. Funct. Biomater., 13.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|