Green Synthesis of Blue-Emitting Graphene Oxide Quantum Dots for In Vitro CT26 and In Vivo Zebrafish Nano-Imaging as Diagnostic Probes

Abstract

Graphene oxide quantum dots (GOQDs) are prepared using black carbon as a feedstock and H2O2 as a green oxidizing agent in a straightforward and environmentally friendly manner. The process adopted microwave energy and only took two minutes. The GOQDs are 20 nm in size and have stable blue fluorescence at 440 nm. The chemical characteristics and QD morphology were confirmed by thorough analysis using scanning electron microscope (SEM), transmission electron microscope (TEM), atomic force microscope (AFM), Fourier transmission infra-red (FT-IR), and X-ray photoelectron spectroscopy (XPS). The biocompatibility test was used to evaluate the toxicity of GOQDs in CT26 cells in vitro and the IC50 was found to be 200 µg/mL with excellent survival rates. Additional in vivo toxicity assessment in the developing zebrafish (Danio rerio) embryo model found no observed abnormalities even at a high concentration of 400 μg/mL after 96 h post fertilization. The GOQDs luminescence was also tested both in vitro and in vivo. They showed excellent internal distribution in the cytoplasm, cell nucleus, and throughout the zebrafish body. As a result, the prepared GOQDs are expected to be simple and inexpensive materials for nano-imaging and diagnostic probes in nanomedicine.