Affiliation:
1. Marine Biology Department, The Leon H. Charney School of Marine Sciences, University of Haifa, Mt. Carmel, Haifa 3103301, Israel
2. Central Fish Health Laboratory, Department of Fisheries and Aquaculture, Ministry of Agriculture and Rural Development, Nir David 1080300, Israel
Abstract
Myxozoa, a unique group of obligate endoparasites within the phylum Cnidaria, can cause emerging diseases in wild and cultured fish populations. Recently, the myxozoan Myxobolus bejeranoi has been identified as a prevalent pathogen infecting the gills of cultured hybrid tilapia, leading to systemic immune suppression and considerable mortality. Here, we employed a proteomic approach to examine the impact of M. bejeranoi infection on fish gills, focusing on the structure of the granulomata, or cyst, formed around the proliferating parasite to prevent its spread to surrounding tissue. Enrichment analysis showed increased immune response and oxidative stress in infected gill tissue, most markedly in the cyst’s wall. The intense immune reaction included a consortium of endopeptidase inhibitors, potentially combating the myxozoan arsenal of secreted proteases. Analysis of the cyst’s proteome and histology staining indicated that keratin intermediate filaments contribute to its structural rigidity. Moreover, we uncovered skin-specific proteins, including a grainyhead-like transcription factor and a teleost-specific S100 calcium-binding protein that may play a role in epithelial morphogenesis and cysts formation. These findings deepen our understanding of the proteomic elements that grant the cyst its distinctive nature at the critical interface between the fish host and myxozoan parasite.
Funder
Israel Science Foundation
University of Haifa
Reference85 articles.
1. El-Sayed, A.-F.M. (2020). Tilapia Culture, Elsevier. [2nd ed.].
2. Hulata, G. (2014). Tilapias—Biology and Culture, Ma’arechet Publishing House.
3. Effect of different management practices on water quality of intensive tilapia culture systems in Israel;Milstein;Aquac. Int.,2001
4. Use and exchange of genetic resources of Nile tilapia (Oreochromis niloticus);Eknath;Rev. Aquac.,2009
5. Trends of aquaculture production and trade: Carp, tilapia, and shrimp;Miao;Asian Fish. Sci.,2020