Identification and Evolutionary Analysis of the Auxin Response Factor (ARF) Family Based on Transcriptome Data from Caucasian Clover and Analysis of Expression Responses to Hormones
-
Published:2023-10-19
Issue:20
Volume:24
Page:15357
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Jiang Jingwen1,
Wang Zicheng1,
Chen Zirui1,
Wu Yuchen1,
Mu Meiqi1,
Nie Wanting1,
Zhao Siwen1,
Cui Guowen1,
Yin Xiujie1
Affiliation:
1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
Abstract
Caucasian clover (Trifolium ambiguum M. Bieb.) is an excellent perennial plant in the legume family Fabaceae, with a well-developed rhizome and strong clonal growth. Auxin is one of the most important phytohormones in plants and plays an important role in plant growth and development. Auxin response factor (ARF) can regulate the expression of auxin-responsive genes, thus participating in multiple pathways of auxin transduction signaling in a synergistic manner. No genomic database has been established for Caucasian clover. In this study, 71 TaARF genes were identified through a transcriptomic database of Caucasian clover rhizome development. Phylogenetic analysis grouped the TaARFs into six (1–6) clades. Thirty TaARFs contained a complete ARF structure, including three relatively conserved regions. Physical and chemical property analysis revealed that TaARFs are unstable and hydrophilic proteins. We also analyzed the expression pattern of TaARFs in different tissues (taproot, horizontal rhizome, swelling of taproot, rhizome bud and rhizome bud tip). Quantitative real–time RT–PCR revealed that all TaARFs were responsive to phytohormones (indole-3-acetic acid, gibberellic acid, abscisic acid and methyl jasmonate) in roots, stems and leaves. These results helped elucidate the role of ARFs in responses to different hormone treatments in Caucasian clover.
Funder
Research and the Natural Science Foundation of Heilongjiang Province of China
the National Natural Science Foundation of China
the “Double First-Class” Discipline Collaborative Innovation Achievement Project of Heilongjiang Province of China
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference72 articles.
1. Effects of degrees of grass competition on spreading of Kura clover;Kim;Grassl. Sci.,2017
2. Impact of Incorporation, Mulch, and Root Coating on the Establishment of Kura Clover from Rhizome Segments on Mine Spoils in Southeastern Kentucky;Teutsch;Forage Grazinglands,2004
3. Vegetative propagation of kura clover: A field-scale test;Baker;Can. J. Plant Sci.,2012
4. Combining Kura Clover with Forage Legumes and Grasses to Optimize Pasture Forage Legume Content;Riday;Agron. J.,2012
5. Xiujie, Y. (2004). Study on the Adaptation and Utilization of Trifolium ambiguum Bieb. [Master’s Thesis, Inner Mongolia Agricultural University].