Evidence of Immunoproteasome Expression Onset in the Formative State of Pluripotency in Mouse Cells

Author:

Kriger Daria1ORCID,Podenkova Uliana I.1ORCID,Bakhmet Evgeny I.1ORCID,Potapenko Evgenii2ORCID,Ivanova Elena1ORCID,Tomilin Alexey N.1ORCID,Tsimokha Anna S.1ORCID

Affiliation:

1. Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, 194064 St. Petersburg, Russia

2. Institute of Evolution, University of Haifa, Haifa 3498838, Israel

Abstract

Embryonic stem cells (ESCs) are remarkable for the high activity level of ubiquitin–proteasome system—the molecular machinery of protein degradation in the cell. Various forms of the proteasome complexes comprising different subunits and interacting regulators are responsible for the substrate selectivity and degradation. Immunoproteasomes are amongst these forms which play an important role in antigen presentation; however, a body of recent evidence suggests their functions in pluripotent stem cells. Previous studies have established three consecutive phases of pluripotency, featured by epiblast cells and their cultured counterparts: naïve, formative, and primed phase. In this work, we report that immunoproteasomes and their chaperone co-regulators are suppressed in the naïve state but are readily upregulated in the formative phase of the pluripotency continuum, featured by epiblast-like cells (EpiLCs). Our data lay ground for the further investigation of the biological functions of immunoproteasome in the regulation of proteostasis during early mammalian development.

Funder

Russian Science Foundation

Publisher

MDPI AG

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