15N Natural Abundance, Nitrogen and Carbon Pools in Soil-Sorghum System Amended with Natural and NH4+-Enriched Zeolitites

Abstract

The use of rocks containing high amounts of natural zeolites (zeolitites) as soil amendment has been found as a valuable method for increasing agriculture sustainability. However, the potentialities and the effects of zeolitites on the biogeochemical cycles of nitrogen (N) and carbon (C) have still not been clearly addressed in the literature. The objective of this study was therefore to investigate the N and C pools and 15N distribution in an agricultural soil amended with both natural and NH4+-enriched zeolitites with the aim of understanding their effects on the soil-plant system, during sorghum cultivation, under fertilization reductions. Zeolitites were applied to an agricultural soil both at natural state (5 and 15 kg m−2) and in an enriched state with NH4+ ions from pig slurry (7 kg m−2). Both zeolitites at natural and enriched state increased soil cation exchange capacity and affected microbial biomass, causing an initial decrease of microbial C and N and then a possible increase of fungal population. N-NO3− content was lower in natural zeolitite treatments, that lead to a lower NO3− availability for denitrifying bacteria. Zeolitites slightly affected the fixed N-NH4+ pool. δ15N turnover indicated that N from NH4+-enriched zeolitites remained in the soil until the growing season and that fertilizers partially substituted the fixed pool. Leaf δ15N content indicated that plants assimilated N from NH4+-enriched zeolitites and evidenced a higher fertilization recovery in natural zeolitite treatments. Organic C tended to be higher in all zeolitite treatment rhizospheres. In soils amended with zeolitites at natural state (at both application rates) sorghum yield was similar (+3.7%) to that obtained in the control while it was higher (+13.9%) in the plot amended with NH4+-enriched zeolitites.