The Diversity of Fungi Involved in Damage to Japanese Quince

Abstract

In recent years, Japanese quince (Chaenomeles japonica) plantations in Latvia have increased. Interest in breeding Japanese quince is also known in other European countries and Russia. The occurrence and harmfulness of fungal diseases have become increasingly significant. However, there is a lack of overall information in the literature about the diversity of fungi afflicting C. japonica. In our study, we aimed to determine the diversity of fungi associated with C. japonica in Latvia, with the possibility of identifying the fungi that are most characteristically associated with certain parts of the plant. Our research was conducted from 2017 to 2019 in eight Japanese quince plantations in Latvia. Samples of plant parts with disease symptoms were collected. Pure cultures of fungi were obtained and identified using ITS region sequencing. We determined the relative density of identified genera of fungi, which were grouped using hierarchical cluster analysis depending on the plant part from which they were found. Various disease-like symptoms were observed and described. A total of 538 isolates of fungi were obtained that belong to 36 genera and represent different ecological niches. Fusarium, Alternaria, Botrytis, and Sarocladium were the genera most frequently found during our study. The number of identified cases of fungal genera differed depending on the part of the plant from which the fungi were obtained. However, it is not possible to relate a specific genus of fungus to only one certain part of a plant. Further research is needed to clarify the pathogenicity of detected fungi and the composition of species in the detected genera of fungi.