Prediction of Heterosis for Agronomic Traits in Half-Diallel Cross of Rice (Oryza sativa L.) under Drought Stress Using Microsatellite Markers

Abstract

Rice breeders are encouraged to classify potential F1-hybrids without crossing all viable mixtures by testing depending on genetic distance due to lack of labor and time in yield trials. The goals of this research were to establish heterosis and investigate the relationship between genomic distance and agronomic attributes under drought. Half-diallel mating design, 28 F1′s and 8 parents were evaluated under drought and genotyped using 11 microsatellite markers. In total, 39 alleles were detected. Results indicated that the greatest heterotic effects for grain yield were observed in Sakha 103 × Sakha 104 and GZ7576-10-3-2-1 × Giza 179, which gave 29.32–22.57% heterosis, respectively. Heterosis for grain yield in these crosses occurred as a rise in panicle weight, filled grains per panicle, low sterility and 100-grain weight. Correlations of marker-based genetic distance with mid-parent heterosis were positively and significantly correlated with sterility percentage (r = 0.390 *, p < 0.05). However, better-parent heterosis was positively and significantly correlated with sterility percentage (r = 0.352 *, p < 0.05) and grain yield per plant (r = 0.345 *, p < 0.05). Associations indicate that high grain yield and low sterility of rice crosses can be expected from microsatellite marker-defined distances of parents. This study indicated that genetic distance is very effective for heterosis prediction in breeding programs.