Author:
Gong Siqi,Gautam Seijal,Coneglio Joshua D.,Scinto Hanna B.,Ruprecht Ruth M.
Abstract
When constructing isogenic recombinant IgM–IgG pairs, we discovered that μ heavy chains strongly prefer partnering with λ light chains for optimal IgM expression in transiently cotransfected Expi293 cells. When μ chains were paired with κ light chains, IgM yields were low but increased by logs—up to 20,000 X—by using λ chains instead. Switching light chains did not alter epitope specificity. For dimeric IgA2, optimal expression involved pairing with λ chains, whereas light-chain preference varied for other immunoglobulin classes. In summary, recombinant IgM production can be drastically increased by using λ chains, an important finding in the use of IgM for mucosal immunoprophylaxis.
Funder
National Institute of Health
Subject
Drug Discovery,Immunology,Immunology and Allergy
Cited by
1 articles.
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