Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids-Reference-Cited by-同舟云学术

Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids

Published:2023-03-06 Issue:2 Volume:6 Page:25
ISSN:2409-9279
Container-title:Methods and Protocols
language:en
Short-container-title:MPs

Author:

Asad Nadeem¹²,Smith Emily¹³⁴⁵^ORCID,Shakya Sudeep¹,Stegman Sutton¹,Timmons Lisa¹

Affiliation:

1. Department of Molecular Biosciences, The University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USA

2. Department of Biochemistry and Molecular Medicine, 64 Medical Center Drive, University of West Virginia Health Science Center, Morgantown, WV 26506, USA

3. Department of Neurobiology, Physiology & Behavior, University of California, Davis, CA 95618, USA

4. Department of Psychiatry and Behavioral Sciences, University of California, Davis, CA 95618, USA

5. Center for Neuroscience, University of California, Davis, CA 95618, USA

Abstract

We describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of difficult-to-obtain plasmids. Sustainability is increased when agarose is used ten times over by virtue of a thorough recycling regimen. The workflow optimizes workspaces and standardizes lab practices for handling potentially hazardous waste, minimizing environmental harm. Safety, efficiency, and sustainability improve laboratory productivity, help minimize environmental contamination, and increase cost-effectiveness.

Funder

University of Kansas

Publisher

MDPI AG

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Structural Biology,Biotechnology

Link

https://www.mdpi.com/2409-9279/6/2/25/pdf

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