A Diet Enriched with Lacticaseibacillus rhamnosus HN001 and Milk Fat Globule Membrane Alters the Gut Microbiota and Decreases Amygdala GABA a Receptor Expression in Stress-Sensitive Rats
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Published:2023-06-21
Issue:13
Volume:24
Page:10433
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Dalziel Julie E.1ORCID, Zobel Gosia2ORCID, Dewhurst Hilary1, Hurst Charlotte1, Olson Trent1, Rodriguez-Sanchez Raquel2, Mace Louise1, Parkar Nabil1, Thum Caroline1ORCID, Hannaford Rina3, Fraser Karl1ORCID, MacGibbon Alastair4ORCID, Bassett Shalome A.4, Dekker James4, Anderson Rachel C.1, Young Wayne1
Affiliation:
1. Smart Foods & Bioproducts, AgResearch, Palmerston North 4442, New Zealand 2. Ethical Agriculture, AgResearch, Hamilton 3240, New Zealand 3. Digital Agriculture, AgResearch, Palmerston North 4442, New Zealand 4. Fonterra Research and Development Centre Co., Ltd., Palmerston North 4442, New Zealand
Abstract
Brain signalling pathways involved in subclinical anxiety and depressed mood can be modulated via the gut brain axis (GBA), providing the potential for diet and dietary components to affect mood. We investigated behavioural, physiological and gut microbiome responses to the Lacticaseibacillus rhamnosus strain HN001 (LactoB HN001™), which has been shown to reduce postpartum anxiety and depression, and a milk fat globule membrane-enriched product, Lipid 70 (SurestartTM MFGM Lipid 70), which has been implicated in memory in stress-susceptible Wistar Kyoto rats. We examined behaviour in the open field, elevated plus maze and novel object recognition tests in conjunction with the expression of host genes in neuro-signalling pathways, and we also assessed brain lipidomics. Treatment-induced alterations in the caecal microbiome and short-chain fatty acid (SCFA) profiles were also assessed. Neither ingredient induced behavioural changes or altered the brain lipidome (separately or when combined). However, with regard to brain gene expression, the L. rhamnosus HN001 + Lipid 70 combination produced a synergistic effect, reducing GABAA subunit expression in the amygdala (Gabre, Gat3, Gabrg1) and hippocampus (Gabrd). Treatment with L. rhamnosus HN001 alone altered expression of the metabotropic glutamate receptor (Grm4) in the amygdala but produced only minor changes in gut microbiota composition. In contrast, Lipid 70 alone did not alter brain gene expression but produced a significant shift in the gut microbiota profile. Under the conditions used, there was no observed effect on rat behaviour for the ingredient combination. However, the enhancement of brain gene expression by L. rhamnosus HN001 + Lipid 70 implicates synergistic actions on region-specific neural pathways associated with fear, anxiety, depression and memory. A significant shift in the gut microbiota profile also occurred that was mainly attributable to Lipid 70.
Funder
Fonterra Ltd. Ministry of Business, Innovation & Employment, New Zealand
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Cited by
1 articles.
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