Affiliation:
1. Dipartimento Medicina Veterinaria, Università degli Studi di Sassari, 07100 Sassari, Italy
2. Centre de Recerca Agrigenòmica (CRAG), Campus Universitat Autònoma de Barcelona, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
3. Dipartimento Medicina Veterinaria, Università degli Studi di Bari Aldo Moro, 70121 Bari, Italy
Abstract
This work aimed to assess the variability of casein genes in a population of 153 bucks and 825 lactating does of the Sarda breed, and to perform association analysis between polymorphic sites and milk yield and composition traits. To genotype the casein genes, we chose an SNP panel including 44 SNPs mapping to the four casein genes CSN1S1, CSN2, CSN1S2, and CSN3. Genotyping (made by KASP™ genotyping assay, based on competitive allele-specific PCR) revealed the high variability of the Sarda goat, and haplotype analysis revealed linkage disequilibrium (LD) between CSN1S1 and CSN2 genes, in addition to two LD blocks within the CSN1S2 and two LD blocks within the CSN3 gene, in bucks and does. Association analysis revealed that variability at all four casein genes was associated with milk protein content, total solids, and milk energy. The three Ca-sensitive casein genes were associated with lipid content, and CSN1S2 showed a unique pattern, with intron variants associated with milk yield, in addition to milk pH, NaCl, and SCS (Somatic Cell Score). This information might prove useful in selection schemes and in future investigations aiming to better understand the biology of lactation, and the direct link between genotype and phenotype.
Funder
Regional Government of Sardinia
Subject
General Veterinary,Animal Science and Zoology
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