Prevalence, Molecular Characteristics and Virulence Identification of Bovine Parainfluenza Virus Type 3 in China-Reference-Cited by-同舟云学术

Prevalence, Molecular Characteristics and Virulence Identification of Bovine Parainfluenza Virus Type 3 in China

Published:2024-03-05 Issue:3 Volume:16 Page:402
ISSN:1999-4915
Container-title:Viruses
language:en
Short-container-title:Viruses

Author:

Xu Xiaowen¹²³,Zhao Wanyue¹²³,Xiang Zhijie¹²³,Wang Chen¹²³,Qi Mingpu¹²³,Zhang Sen¹²³,Geng Yuanchen¹²³,Zhao Yuhao¹²³,Yang Kaihui¹²³,Zhang Yanan¹²³,Guo Aizhen¹²³⁴,Chen Yingyu¹²³⁴^ORCID

Affiliation:

1. National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China

2. Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China

3. Key Laboratory of Development of Veterinary Diagnostic Products, Huazhong Agricultural University, Ministry of Agriculture and Rural Affairs, Wuhan 430070, China

4. Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China

Abstract

Bovine parainfluenza virus type 3 (BPIV-3) is one of the major pathogens of the bovine respiratory disease complex (BRDC). BPIV-3 surveillance in China has been quite limited. In this study, we used PCR to test 302 cattle in China, and found that the positive rate was 4.64% and the herd-level positive rate was 13.16%. Six BPIV-3C strains were isolated and confirmed by electron microscopy, and their titers were determined. Three were sequenced by next-generation sequencing (NGS). Phylogenetic analyses showed that all isolates were most closely related to strain NX49 from Ningxia; the genetic diversity of genotype C strains was lower than strains of genotypes A and B; the HN, P, and N genes were more suitable for genotyping and evolutionary analyses of BPIV-3. Protein variation analyses showed that all isolates had mutations at amino acid sites in the proteins HN, M, F, and L. Genetic recombination analyses provided evidence for homologous recombination of BPIV-3 of bovine origin. The virulence experiment indicated that strain Hubei-03 had the highest pathogenicity and could be used as a vaccine candidate. These findings apply an important basis for the precise control of BPIV-3 in China.

Funder

The National Key Research and Development Program of China

Publisher

MDPI AG

Link

https://www.mdpi.com/1999-4915/16/3/402/pdf

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