Abstract
Lipids are the fundamental components of cell membranes and they play a significant role in their integrity and fluidity. The alteration in lipid composition of membranes has been reported to be a major response to abiotic environmental stresses. Seasonal dynamics of membrane lipids in the shoots of Ephedra monosperma J.G. Gmel. ex C.A. Mey. growing in natural conditions of permafrost ecosystems was studied using HPTLC, GC-MS and ESI-MS. An important role of lipid metabolism was established during the autumn-winter period when the shoots of the evergreen shrub were exposed to low positive (3.6 °C), negative (−8.3 °C) and extremely low temperatures (−38.4 °C). Maximum accumulation of phosphatidic acid (PA), the amount of which is times times greater than the sum of phosphatidylcholine and phosphatidylethanolamine (PC + PE) was noted in shoots of E. monosperma in the summer-autumn period. The autumn hardening period (3.6 °C) is accompanied by active biosynthesis and accumulation of membrane lipids, a decrease of saturated 34:1 PCs, 34:1 PEs and 34:1 PAs, and an increase in unsaturated long-chain 38:5 PEs, 38:6 PEs, indicating that the adaptation of E. monosperma occurs not at the level of lipid classes but at the level of molecular species. At a further decrease of average daily air temperature in October (−8.3 °C) a sharp decline of PA level was registered. At an extreme reduction of environmental temperature (−38.4 °C) the content of non-bilayer PE and PA increases, the level of unsaturated fatty acids (FA) rises due to the increase of C18:2(Δ9,12) and C18:3(Δ9,12,15) acids and the decrease of C16:0 acids. It is concluded that changes in lipid metabolism reflect structural and functional reorganization of cell membranes and are an integral component of the complex process of plant hardening to low temperatures, which contributes to the survival of E. monosperma monocotyledonous plants in the extreme conditions of the Yakutia cryolithozone.
Funder
President of the Russian Federation for State Support of Young Russian Scientists—Candidates of Sciences and Doctors of Sciences of the Russian Federation
Russian Science Foundation
Ministry of Education and Science of the Russian Federation
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference45 articles.
1. Cano-Ramirez, D.L., Carmona-Salazar, L., Morales-Cedillo, F., Ramírez-Salcedo, J., Cahoon, E.B., and Gavilanes-Ruíz, M. (2021). Plasma Membrane Fluidity: An Environment Thermal Detector in Plants. Cells, 10.
2. Plant adaptation to frequent alterations between high and low temperatures: Remodelling of membrane lipids and maintenance of unsaturation levels;Zheng;Plant Cell Environ.,2011
3. Differential remodeling of the lipidome during cold acclimation in natural accessions of Arabidopsis thaliana;Degenkolbe;Plant J.,2012
4. The conserved theme of ribosome hibernation: From bacteria to chloroplasts of plants;Willmund;Biol. Chem.,2019
5. Los, D.A. (2014). Desaturazy Zhirnykh Kislot [Fatty Acid Desaturases], Nauchnyj Mir.
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献