Affiliation:
1. School of Life Sciences, Chongqing University, Chongqing 401331, China
2. Chongqing Key Laboratory of Biology and Genetic Breeding for Tuber and Root Crops, Chongqing 400716, China
3. College of Agronomy, Xichang University, Xichang 615013, China
Abstract
Phytophthora infestans, the notorious pathogen of potato late blight, leads to a severe decline in potato yields and even harvest failure. We isolated 201 endophytic isolates from healthy root tissues of potatoes, among which 41 showed strong antagonistic activity against P. infestans. Further, the tolerance to stress and the potential application against potato late blight of these antagonistic isolates were tested. Most of them were extremely tolerant to stresses such as acid–alkali, temperature, UV, salt, and heavy metal stress. However, some antagonistic isolates with excellent stress tolerance might be pathogenic to potatoes. Combining the screening results, a total of 14 endophytes had excellent comprehensive performance in all the tests. In this paper, the endophyte 6-5 was selected among them for the preliminary exploration of the anti-oomycete mechanism. Analysis of the 16S rDNA sequence revealed that 6-5 had a high homology to the corresponding sequence of Bacillus velezensis (99.72%) from the NCBI database. Endophyte 6-5 significantly inhibited the mycelial growth of P. infestans, with an inhibition rate of over 90% in vitro assays, and deformed the hyphal phenotype of P. infestans. In addition, endophyte 6-5 could secrete protease and cellulase, and produce antagonistic substances with high thermal stability, which might be helpful to its antagonistic activity against P. infestans. Furthermore, it was demonstrated that 6-5 had the ability to improve the resistance of potato tubers to late blight. In short, our study described the process of isolating and screening endophytes with antagonistic activity against P. infestans from potato roots, and further explored the potential of biocontrol candidate strain 6-5 in potato late blight control.
Funder
National Key Research and Development Program of China
Chongqing Technology Innovation and Application Development Project
Scientific Research Project of Chongqing Ecological Environment Bureau
Open Project Program of Panxi Crops Research and Utilization Key Laboratory of Sichuan Province
Fundamental Research Funds for the Central Universities
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Cited by
6 articles.
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