Do Antimonite and Silicon Share the Same Root Uptake Pathway by Lsi1 in Sorghum bicolor L. Moench?

Author:

Vidya Chirappurathu Sukumaran Nair1,Shetty Rajpal12,Bokor Boris23,Fialová Ivana1,Luxová Miroslava1,Jašková Katarína1,Vaculík Marek12

Affiliation:

1. Institute of Botany, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Dúbravská cesta 14, SK-845 23 Bratislava, Slovakia

2. Department of Plant Physiology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynska dolina B2, Ilkovicova 6, SK-842 15 Bratislava, Slovakia

3. Comenius University Science Park, SK-841 04 Bratislava, Slovakia

Abstract

A study was conducted to further develop our understanding of antimony (Sb) uptake in plants. Unlike other metal(loid)s, such as silicon (Si), the mechanisms of Sb uptake are not well understood. However, SbIII is thought to enter the cell via aquaglyceroporins. We investigated if the channel protein Lsi1, which aids in Si uptake, also plays a role in Sb uptake. Seedlings of WT sorghum, with normal silicon accumulation, and its mutant (sblsi1), with low silicon accumulation, were grown in Hoagland solution for 22 days in the growth chamber under controlled conditions. Control, Sb (10 mg Sb L−1), Si (1mM) and Sb + Si (10 mg Sb L−1 + 1 mM Si) were the treatments. After 22 days, root and shoot biomass, the concentration of elements in root and shoot tissues, lipid peroxidation and ascorbate levels, and relative expression of Lsi1 were determined. When mutant plants were exposed to Sb, they showed almost no toxicity symptoms compared to WT plants, indicating that Sb was not toxic to mutant plants. On the other hand, WT plants had decreased root and shoot biomass, increased MDA content and increased Sb uptake compared to mutant plants. In the presence of Sb, we also found that SbLsi1 was downregulated in the roots of WT plants. The results of this experiment support the role of Lsi1 in Sb uptake in sorghum plants.

Funder

Slovak Research and Development Agency

Slovak Academy of Sciences

VEGA

European Cooperation in Science and Technology

Publisher

MDPI AG

Subject

Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics

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