Characterization of Chlorophyll Fluorescence and Antioxidant Defense Parameters of Two Gracilariopsis lemaneiformis Strains under Different Temperatures

Abstract

In this study, two Gracilariopsis lemaneiformis strains—the wild type and a green-pigmented mutant—were cultured at three temperatures (8, 20, and 30 °C) for 7 days to explore their temperature tolerance using photosynthetic performance and antioxidant defense parameters. When the two strains of G. lemaneiformis were separately cultured at 30 °C, the fast chlorophyll fluorescence intensity of the wild type decreased, whereas the green mutant showed no significant change. The decrease in the performance index on absorption basis value under heat stress was lower in the green mutant than in the wild type. In addition, the green mutant had stronger antioxidant activity at 30 °C. Furthermore, a greater decrease in the values of maximum photochemical quantum yield and performance index on an absorption basis in the green mutant indicated that it had a greater degree of inhibition of photosynthetic performance under low temperatures. However, the green mutant produced less reactive oxygen species under low temperatures, suggesting that the antioxidant potential of the green mutant might be higher. In conclusion, the green mutant exhibited heat tolerance and could recover from low-temperature damage; therefore, it has the potential for large-scale cultivation.