Abstract
Invasive tree species change biodiversity, nutrient cycles, and ecosystem services, and can turn native ecosystems into novel ecosystems determined by invaders. In the acclimatization and invasion of alien tree species, the crucial role is played by ectomycorrhizal (ECM) fungi. We tested ECM fungi associated with Quercus rubra and Carya trees that are alien to Europe. Quercus rubra is among the most invasive tree species in Europe, and the Carya species are not considered invasive. Both form ectomycorrhizal symbiosis, and in their native range in North America, coexist in oak-hickory forests. Six study stands were located in Kórnik Arboretum: three for Q. rubra and three for Carya trees. Ectomycorrhizal fungi were assessed by molecular identification of ECM roots. We identified 73 ECM fungal taxa of 23 ECM phylogenetic lineages. All identified ECM fungi were native to Europe. Similar richness but different composition of ECM taxa were found on Q. rubra and Carya roots. Phylogenetic lineages /tomentella-thelephora, /russula-lactarius, and /genea-humaria were most abundant on both Carya and Q. rubra roots. Lineages /tuber-helvella and /entoloma were abundant only on Carya, and lineages /pisolithus-scleroderma and /cortinarius were abundant only on Q. rubra roots. Analysis of similarities revealed a significant difference in ectomycorrhizal assemblages between invasive Q. rubra and non-invasive Carya. Highlights: (1) under common garden conditions, ECM taxa richness was similar on Q. rubra and Carya roots; (2) ECM taxa composition differed between invasive Q. rubra and non-invasive Carya; (3) high abundance of long-distance exploration type (lineages from Boletales) was on Q. rubra; and (4) high abundance of short-distance exploration type (e.g., /tuber-helvella) was on Carya.