Affiliation:
1. School of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China
2. Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region, Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China
Abstract
The spectral composition of light influences the biosynthesis of flavonoids in many plants. However, the detailed composition of flavonoids and anthocyanins and the molecular basis for their biosynthesis in strawberry fruits under two light-quality treatments, red light supplemented with blue light (RB) and ultraviolet B (UVB) irradiation, remain unclear. In this study, the content of flavonoids and anthocyanins was significantly increased in strawberry fruits under RB light and UVB, respectively. The content of flavonoids and anthocyanins in strawberry fruits under UVB light was dramatically higher than that in strawberry fruits irradiated with RB light, and a total of 518 metabolites were detected by means of LC-MS/MS analysis. Among them, 18 phenolic acids, 23 flavonoids, and 8 anthocyanins were differentially accumulated in the strawberry fruits irradiated with red/blue (RB) light compared to 30 phenolic acids, 46 flavonoids, and 9 anthocyanins in fruits irradiated with UVB. The major genes associated with the biosynthesis of flavonoids and anthocyanins, including structural genes and transcription factors (TFs), were differentially expressed in the strawberry fruits under RB and UVB irradiation, as determined through RNA-seq data analysis. A correlation test of transcriptome and metabolite profiling showed that the expression patterns of most genes in the biosynthesis pathway of flavonoids and anthocyanins were closely correlated with the differential accumulation of flavonoids and anthocyanins. Two TFs, bZIP (FvH4_2g36400) and AP2 (FvH4_1g21210), induced by RB and UVB irradiation, respectively, exhibited similar expression patterns to most structural genes, which were closely correlated with six and eight flavonoids, respectively. These results indicated that these two TFs regulated the biosynthesis of flavonoids and anthocyanins in strawberry fruit under RB light and UVB, respectively. These results provide a systematic and comprehensive understanding of the accumulation of flavonoids and anthocyanins and the molecular basis for their biosynthesis in strawberry fruits under RB light and UVB.
Funder
National Natural Science Foundation of China
China Postdoctoral Science Foundation
National Guidance Foundation for Local Science and Technology Development of China
Reference75 articles.
1. Breeding strawberry (Fragaria × ananassa Duch) to increase fruit nutritional quality;Capocasa;Biofactors,2008
2. Over-expression of Gene FaASR Promotes Strawberry Fruit Coloring;Liu;Hortic. Plant J.,2015
3. Allelic Variation of MYB10 Is the Major Force Controlling Natural Variation in Skin and Flesh Color in Strawberry (Fragaria spp.);Castillejo;Fruit. Plant Cell,2020
4. Preharvest long-term exposure to UV-B radiation promotes fruit ripening and modifies stage-specific anthocyanin metabolism in highbush blueberry;Li;Hortic. Res.,2021
5. Ma, Y., Ma, X., Gao, X., Wu, W., and Zhou, B. (2021). Light Induced Regulation Pathway of Anthocyanin Biosynthesis in Plants. Int. J. Mol. Sci., 22.
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