Response of Tomato Plants, Ailsa Craig and Carotenoid Mutant tangerine, to Simultaneous Treatment by Low Light and Low Temperature

Abstract

Tomato (Solanum lycopersicum L.) plants, wild type Ailsa Craig, and carotenoid mutant tangerine that accumulates prolycopene instead of all-trans-lycopene were exposed to a combined treatment by low light and low temperature for 5 days. The ability of plants to recover from the stress after development for 3 days at control conditions was followed as well. The suffered oxidative stress was evaluated by the extent of pigment content, lipid peroxidation, membrane stability, and H2O2 generation. The level of MDA content under combined treatment in tangerine implies that the mutant demonstrates lower sensitivity to stress in comparison with Ailsa Craig. The oxidative protective strategy of plants was estimated by following the antioxidant and antiradical activity of phenolic metabolites, including anthocyanins, as well as the activities of antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT). Presented results revealed that the oxidative stress was much stronger expressed after exposure of both types of plants to low light combined with low temperature compared to that after treatment with only low light. The most significant antioxidant protection was provided by phenolic substances, including anthocyanins. The lower sensitivity of tangerine plants to low light can be attributed to the higher activity of the antioxidant enzyme CAT.