Comparative Genomics of Three Hybrid-Pathogen Multidrug-Resistant Escherichia coli Strains Isolated from Healthy Donors’ Feces-Reference-Cited by-同舟云学术

Comparative Genomics of Three Hybrid-Pathogen Multidrug-Resistant Escherichia coli Strains Isolated from Healthy Donors’ Feces

Published:2024-08-02 Issue:3 Volume:15 Page:1412-1424
ISSN:2036-7481
Container-title:Microbiology Research
language:en
Short-container-title:Microbiology Research

Author:

Ortega-Enríquez Judith Z.¹²^ORCID,Martínez-de la Peña Claudia¹,Lara-Ochoa Cristina³,Rocha-Gracia Rosa del Carmen¹^ORCID,Barrios-Villa Edwin¹²^ORCID,Arenas-Hernández Margarita M. P.¹

Affiliation:

1. Graduate Program in Microbiology, Centro de Investigación en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla 72570, Mexico

2. Laboratorio de Biología Molecular y Genómica, Departamento de Ciencias Químico Biológicas y Agropecuarias, Universidad de Sonora, Campus Caborca, H. Caborca, Sonora 83621, Mexico

3. Centro de Detección Biomolecular, Benemérita Universidad Autónoma de Puebla, Puebla 72570, Mexico

Abstract

The present study shows the genomic characterization of three pathogenic Escherichia coli hybrid strains. All strains were previously characterized as diarrheagenic pathotypes (DEC), obtained from feces. The three sequenced strains have genes that encode adhesins (fimH and iha) and iron uptake systems (iucC and iutA). Antibiotic resistance genes were also found for fluoroquinolone and aminoglycoside families in the three strains. The presence of genomic islands (GIs) in the sequenced study strains presented 100% identity (Ec-25.2) and 99% identity (Ec-36.1) with previously reported Extraintestinal Pathogenic E. coli (ExPEC) strains. The Ec-36.4 strain shared a 99% identity with GI from the Enterotoxigenic E. coli (ETEC) pathotype of the diarrheagenic E. coli strain. Ec-25.2 belongs to ST69 and harbors a FimH27 variant, while Ec-36.1 and Ec-36.4 belong to ST4238 and share a FimH54 variant. Four incompatibility groups associated with conjugative plasmids were identified (IncFIB, IncF11, IncI1, and IncB/O/K/Z), as well as Insertion Sequences and MITEs elements.

Funder

Universidad de Sonora under the Convocatoria para Apoyo a Proyectos Internos 2023

Vicerrectoría de Investigación y Estudios de Posgrado (VIEP), BUAP

CONAHCYT

Publisher

MDPI AG

Link

https://www.mdpi.com/2036-7481/15/3/95/pdf

Reference90 articles.

1. Erjavec, M.S. (2019). The Universe of Escherichia coli, IntechOpen. Available online: https://www.intechopen.com/books/6970.

2. Escherichia coli diarreogénicos, identificación de patotipos y fenotipos de resistencia antimicrobiana en aislados cubanos;Rev. Cuba. Med. Trop.,2020

3. Torres, A.G. (2016). Escherichia coli in the Americas, Springer.

4. Mecanismos de virulencia de Escherichia coli enteropatógena;Rev. Chil. Infectología,2016

5. Geurtsen, J., De Been, M., Weerdenburg, E., Zomer, A., McNally, A., and Poolman, J. (2022). Genomics and pathotypes of the many faces of Escherichia coli. FEMS Microbiol. Rev., 46.