Transcriptome Analysis Reveals the Mechanism of Exogenous Selenium in Alleviating Cadmium Stress in Purple Flowering Stalks (Brassica campestris var. purpuraria)

Author:

Huang Zhi1ORCID,Meng Shiling1,Huang Juan1,Zhou Wende1,Song Xiaoli1,Hao Peiyao1,Tang Peigen1,Cao Yihan1,Zhang Fen1,Li Huanxiu1,Tang Yi1,Sun Bo1ORCID

Affiliation:

1. College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China

Abstract

In China, cadmium (Cd) stress has a significant role in limiting the development and productivity of purple flowering stalks (Brassica campestris var. purpuraria). Exogenous selenium supplementation has been demonstrated in earlier research to mitigate the effects of Cd stress in a range of plant species; nevertheless, the physiological and molecular processes by which exogenous selenium increases vegetable shoots’ resistance to Cd stress remain unclear. Purple flowering stalks (Brassica campestris var. purpuraria) were chosen as the study subject to examine the effects of treatment with sodium selenite (Na2SeO3) on the physiology and transcriptome alterations of cadmium stress. Purple flowering stalk leaves treated with exogenous selenium had higher glutathione content, photosynthetic capacity, and antioxidant enzyme activities compared to the leaves treated with Cd stress alone. Conversely, the contents of proline, soluble proteins, soluble sugars, malondialdehyde, and intercellular CO2 concentration tended to decrease. Transcriptome analysis revealed that 2643 differentially expressed genes (DEGs) were implicated in the response of exogenous selenium treatment to Cd stress. The metabolic pathways associated with flavonoid production, carotenoid synthesis, glutathione metabolism, and glucosinolate biosynthesis were among those enriched in these differentially expressed genes. Furthermore, we discovered DEGs connected to the production route of glucosinolates. This work sheds fresh light on how purple flowering stalks’ tolerance to cadmium stress is improved by exogenous selenium.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Sichuan Province

Central Leading Local Science and Technology Development Project

Joint research on mustard breeding in Sichuan Province

Project of New Varieties Breeding of Sichuan Vegetable Innovation Team

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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