Affiliation:
1. Zhengzhou Research Base, National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China
2. Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China
Abstract
Dramatic shifts in global climate have intensified abiotic and biotic stress faced by plants. Plant microRNAs (miRNAs)—20–24 nucleotide non-coding RNA molecules—form a key regulatory system of plant gene expression; playing crucial roles in plant growth; development; and defense against abiotic and biotic stress. Moreover, they participate in cross-kingdom communication. This communication encompasses interactions with other plants, microorganisms, and insect species, collectively exerting a profound influence on the agronomic traits of crops. This article comprehensively reviews the biosynthesis of plant miRNAs and explores their impact on plant growth, development, and stress resistance through endogenous, non-transboundary mechanisms. Furthermore, this review delves into the cross-kingdom regulatory effects of plant miRNAs on plants, microorganisms, and pests. It proceeds to specifically discuss the design and modification strategies for artificial miRNAs (amiRNAs), as well as the protection and transport of miRNAs by exosome-like nanovesicles (ELNVs), expanding the potential applications of plant miRNAs in crop breeding. Finally, the current limitations associated with harnessing plant miRNAs are addressed, and the utilization of synthetic biology is proposed to facilitate the heterologous expression and large-scale production of miRNAs. This novel approach suggests a plant-based solution to address future biosafety concerns in agriculture.
Funder
National Natural Science Foundation of China
China Postdoctoral Science Foundation
Innovation Program of Chinese Academy of Agricultural Sciences
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference179 articles.
1. MicroRNAs: Genomics, biogenesis, mechanism, and function;Bartel;Cell,2004
2. Biogenesis, turnover, and mode of action of plant microRNAs;Rogers;Plant Cell,2013
3. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14;Lee;Cell,1993
4. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans;Reinhart;Nature,2000
5. Vella, M.C., and Slack, F.J. (2005). C. Elegans microRNAs, WormBook.