Mass Spectrometry Imaging Combined with Sparse Autoencoder Method Reveals Altered Phosphorylcholine Distribution in Imipramine Treated Wild-Type Mice Brains
-
Published:2024-07-21
Issue:14
Volume:25
Page:7969
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Rahman Md Foyzur1ORCID, Islam Ariful1ORCID, Islam Md. Monirul1, Mamun Md. Al12ORCID, Xu Lili1ORCID, Sakamoto Takumi12, Sato Tomohito1ORCID, Takahashi Yutaka12, Kahyo Tomoaki13, Aoyagi Satoka4ORCID, Kaibuchi Kozo5, Setou Mitsutoshi16ORCID
Affiliation:
1. Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Chuo-ku, Hamamatsu 431-3192, Shizuoka, Japan 2. Preppers Co., Ltd., 1-20-1 Handayama, Chuo-ku, Hamamatsu 431-3192, Shizuoka, Japan 3. Quantum Imaging Laboratory, Division of Research and Development in Photonics Technology/International Mass Imaging and Spatial Omics Center, Institute of Photonics Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Chuo-ku, Hamamatsu 431-3192, Shizuoka, Japan 4. Faculty of Science and Technology, Seikei University, 3-3-1 Kichijoji-kitamachi, Musashino-shi 180-8633, Tokyo, Japan 5. Division of Cell Biology, International Center for Brain Science, Fujita Health University, Toyoake 470-1192, Aichi, Japan 6. International Mass Imaging and Spatial Omics Center, Institute of Photonics Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Chuo-ku, Hamamatsu 431-3192, Shizuoka, Japan
Abstract
Mass spectrometry imaging (MSI) is essential for visualizing drug distribution, metabolites, and significant biomolecules in pharmacokinetic studies. This study mainly focuses on imipramine, a tricyclic antidepressant that affects endogenous metabolite concentrations. The aim was to use atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI)-MSI combined with different dimensionality reduction methods to examine the distribution and impact of imipramine on endogenous metabolites in the brains of treated wild-type mice. Brain sections from both control and imipramine-treated mice underwent AP-MALDI-MSI. Dimensionality reduction methods, including principal component analysis, multivariate curve resolution, and sparse autoencoder (SAE), were employed to extract valuable information from the MSI data. Only the SAE method identified phosphorylcholine (ChoP) as a potential marker distinguishing between the control and treated mice brains. Additionally, a significant decrease in ChoP accumulation was observed in the cerebellum, hypothalamus, thalamus, midbrain, caudate putamen, and striatum ventral regions of the treated mice brains. The application of dimensionality reduction methods, particularly the SAE method, to the AP-MALDI-MSI data is a novel approach for peak selection in AP-MALDI-MSI data analysis. This study revealed a significant decrease in ChoP in imipramine-treated mice brains.
Funder
Japan Society for the Promotion of Science Japan Agency for Medical Research and Development Ministry of Education, Culture, Sports, Science and Technology
Reference32 articles.
1. Quantitative Mass Spectrometry Imaging of Biological Systems;Unsihuay;Annu. Rev. Phys. Chem.,2021 2. Detection of Distinct Distributions of Acetaminophen and Acetaminophen-Cysteine in Kidneys up to 10 μm Resolution and Identification of a Novel Acetaminophen Metabolite Using an AP-MALDI Imaging Mass Microscope;Mamun;J. Am. Soc. Mass Spectrom.,2023 3. Islam, A., Sakamoto, T., Zhai, Q., Rahman, M.M., Mamun, M.A., Takahashi, Y., Kahyo, T., and Setou, M. (2022). Application of AP-MALDI Imaging Mass Microscope for the Rapid Mapping of Imipramine, Chloroquine, and Their Metabolites in the Kidney and Brain of Wild-Type Mice. Pharmaceuticals, 15. 4. Islam, M.M., Rahman, M.F., Islam, A., Afroz, M.S., Mamun, M.A., Rahman, M.M., Maniruzzaman, M., Xu, L., Sakamoto, T., and Takahashi, Y. (2024). Elucidating Gender-Specific Distribution of Imipramine, Chloroquine, and Their Metabolites in Mice Kidney Tissues through AP-MALDI-MSI. Int. J. Mol. Sci., 25. 5. Kawashima, T., Aoki, T., Taniike, Y., and Aoyagi, S. (2020). Examination of beauty ingredient distribution in the human skin by time-of-flight secondary ion mass spectrometry. Biointerphases, 15.
|
|