High-Throughput Sequencing of Grapevine in Mexico Reveals a High Incidence of Viruses including a New Member of the Genus Enamovirus
Author:
Diaz-Lara Alfredo1ORCID, Stevens Kristian23, Aguilar-Molina Vivian Hayde1, Fernández-Cortés José Miguel1, Chabacano León Víctor Manuel4, De Donato Marcos1ORCID, Sharma Ashutosh1ORCID, Erickson Teresa M.3, Al Rwahnih Maher3ORCID
Affiliation:
1. School of Engineering and Sciences, Tecnologico de Monterrey, Campus Queretaro, Queretaro 76130, Mexico 2. Departments of Computer Science and Evolution and Ecology, University of California-Davis, Davis, CA 95616, USA 3. Foundation Plant Services, University of California-Davis, Davis, CA 95616, USA 4. Facultad de Química, Universidad Autónoma de Querétaro, Santiago de Queretaro, Queretaro 76010, Mexico
Abstract
This is the first viral metagenomic analysis of grapevine conducted in Mexico. During the summer of 2021, 48 plants displaying virus-like symptoms were sampled in Queretaro, an important grapevine-producing area of Mexico, and analyzed for the presence of viruses via high-throughput sequencing (HTS). The results of HTS were verified by real-time RT-PCR following a standardized testing scheme (Protocol 2010). Fourteen different viruses were identified, including grapevine asteroid mosaic-associated virus (GAMaV), grapevine Cabernet Sauvignon reovirus (GCSV), grapevine fanleaf virus (GFLV), grapevine fleck virus (GFkV), grapevine Pinot gris virus (GPGV), grapevine red globe virus (GRGV), grapevine rupestris stem pitting-associated virus (GRSPaV), grapevine rupestris vein feathering virus (GRVFV), grapevine Syrah virus 1 (GSyV-1), grapevine virus B (GVB), and grapevine leafroll-associated viruses 1, 2, 3, 4 (GLRaV1, 2, 3, 4). Additionally, divergent variants of GLRaV4 and GFkV, and a novel Enamovirus-like virus were discovered. This is the first report of GAMaV, GCSV, GLRaV4, GPGV, GRGV, GRVFV, and GSyV-1 infecting grapevines in Mexico; the impact of these pathogens on production is unknown.
Funder
2021 Tec-BASE Seed Fund for Research Projects
Subject
Virology,Infectious Diseases
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