Variety and Site Drive Salix Mixture Effects on Soil Organic Matter Chemistry and Soil Carbon Accumulation

Abstract

Soil organic matter (SOM) is essential for nutrient cycling and soil carbon (C) accumulation, both of which are heavily influenced by the quality and quantity of plant litter. Since SOM dynamics in relation to plant diversity are poorly understood, we investigated the effects of willow variety and mixture, and site on the soil C stocks, SOM chemical composition and thermal stability. Using pyrolysis-field ionization mass spectrometry (Py-FIMS), a method of stepwise thermal degradation in ultrahigh vacuum combined with soft ionization in a high electric field, followed by mass-spectrometric separation and detection of molecular ions, we analyzed SOM in the top 10 cm of soil from two 7-year-old experimental sites in Germany and Sweden. Monocultures and mixtures of two willow varieties (Salix spp.) belonging to different species were grown at the experimental plots. Overall, site had the strongest effect on SOM quality. The results showed significant variability across sites for willow identity and mixture effects on C accumulation and SOM chemistry. In the German site (Rostock), yearly soil C accumulation was higher (p < 0.05) for variety ‘Loden’ (1.0 Mg C ha−1 year−1) compared to ‘Tora’ (0.5 Mg C ha−1 year−1), whilst in the Swedish site (Uppsala), both varieties exhibited similar soil C accumulation rates of around 0.6 Mg C ha−1 year−1. Willow variety identity significantly affected SOM quality at both sites, while mixing had minor effects. Our findings emphasize the significance of site-specific context and variety and species identity in shaping soil C accumulation in willow plantations.