Developmental Mapping of Hair Follicles in the Embryonic Stages of Cashmere Goats Using Proteomic and Metabolomic Construction
Author:
Gao Yuan1, Duo Lei1, Zhe Xiaoshu1, Hao Lingyun1, Song Weiguo1, Gao Lizhong2, Cai Jun2, Liu Dongjun1
Affiliation:
1. State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China 2. Key Laboratory of Cashmere Materials and Engineering Technology in Inner Mongolia Autonomous Region, Ordos 010090, China
Abstract
The hair follicle (HF) is the fundamental unit for fleece and cashmere production in cashmere goats and is crucial in determining cashmere yield and quality. The mechanisms regulating HF development in cashmere goats during the embryonic period remain unclear. Growing evidence suggests that HF development involves complex developmental stages and critical events, and identifying the underlying factors can improve our understanding of HF development. In this study, samples were collected from embryonic day 75 (E75) to E125, the major HF developmental stages. The embryonic HFs of cashmere goats were subjected to proteomic and metabolomic analyses, which revealed dynamic changes in the key factors and signalling pathways controlling HF development at the protein and metabolic levels. Gene ontology and the Kyoto Encyclopaedia of Genes and Genomes were used to functionally annotate 1784 significantly differentially expressed proteins and 454 significantly differentially expressed metabolites enriched in different HF developmental stages. A joint analysis revealed that the oxytocin signalling pathway plays a sustained role in embryonic HF development by activating the MAPK and Ca2+ signalling pathways, and a related regulatory network map was constructed. This study provides a global perspective on the mechanism of HF development in cashmere goats and enriches our understanding of embryonic HF development.
Funder
Science and Technology Major Project of the Inner Mongolia Autonomous Region of China
Subject
General Veterinary,Animal Science and Zoology
Reference35 articles.
1. Nocelli, C., Cappelli, K., Capomaccio, S., Pascucci, L., Mercati, F., Pazzaglia, I., Mecocci, S., Antonini, M., and Renieri, C. (2020). Shedding light on cashmere goat hair follicle biology: From morphology analyses to transcriptomic landascape. BMC Genom., 21. 2. Construction and functional analysis of ceRNA regulatory network related to the development of secondary hair follicles in Inner Mongolia cashmere goats;Shang;Front. Vet. Sci.,2022 3. A single-cell transcriptome atlas of cashmere goat hair follicle morphogenesis;Ge;Genom. Proteom. Bioinform.,2021 4. Inner Mongolian cashmere goat secondary follicle development regulation research based on mRNA-miRNA co-analysis;Han;Sci. Rep.,2020 5. Wang, S., Li, F., Liu, J., Zhang, Y., Zheng, Y., Ge, W., Qu, L., and Wang, X. (2020). Integrative analysis of methylome and transcriptome reveals the regulatory mechanisms of hair follicle morphogenesis in cashmere goat. Cells, 9.
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