Methodological Principles of Nasal Food Challenge

Author:

Krzych-Fałta Edyta1,Czerwińska Monika E.2ORCID,Białek Sławomir2ORCID,Furmańczyk Konrad3,Samoliński Bolesław4,Grodner Błażej2,Sybilski Adam5,Nowicka Grażyna2ORCID,Wojas Oksana4

Affiliation:

1. Department of Basic Nursing, Medical University of Warsaw, 02-097 Warsaw, Poland

2. Department of Biochemistry and Pharmacogenomics, Medical University of Warsaw, 02-097 Warsaw, Poland

3. Institute of Information Technology, Warsaw University of Life Sciences, 02-776 Warsaw, Poland

4. Department of Prevention of Environmental Hazards, Allergology and Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland

5. 2nd Department of Pediatrics, Centre of Postgraduate Medical Education, 01-813 Warsaw, Poland

Abstract

Thanks to their valuable assessment possibilities (subjective complaints and changes in nasal patency during the examination), nasal provocation tests may serve as an alternative tool for oral food challenges in the future. However, this test requires successive attempts to regulate its methodology in order to develop a standardized lyophilisate form and determine the threshold dose for a positive result. The study objective was to present the methodological foundation for nasal food allergen provocation tests induced by freeze-dried powdered chicken egg whites. A control group of 25 individuals with no history of allergy to chicken eggs or any other allergy was included in the study. Optical rhinometry and visual analog scales were used to assess the response of nasal mucosa to local allergen challenges. Minor variations in nasal flows, as measured by optical rhinometry, were observed in the provocation tests. The mean optical density measurements (as measured regardless of the allergen dose used) varied from positive to negative values and vice versa, e.g., amounting to 0.018 OD (standard deviation 0.095) at 15 min and −0.011 OD (standard deviation 0.090) at 30 min. No significant differences were observed concerning the perceived nasal discomfort using the visual analog scale. Due to the absence of nasal mucosal reactivity, nasal challenge is an excellent methodological tool for implementing food allergen tests.

Funder

National Science Center Miniature-5

Publisher

MDPI AG

Subject

Food Science,Nutrition and Dietetics

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