A Fluorescent Lateral Flow Immunoassay for the Detection of Skeletal Muscle Troponin I in Serum for Muscle Injury Monitoring at the Point of Care-Reference-Cited by-同舟云学术

A Fluorescent Lateral Flow Immunoassay for the Detection of Skeletal Muscle Troponin I in Serum for Muscle Injury Monitoring at the Point of Care

Published:2024-08-08 Issue:8 Volume:14 Page:381
ISSN:2079-6374
Container-title:Biosensors
language:en
Short-container-title:Biosensors

Author:

Tang Deding¹²³,Wu Shuang¹²,Kong Mengqi¹²⁴,Liu Zhaonan¹²⁴,Li Zonghao¹²⁴,Han Ying⁵,Gong Yan⁵,Hu Jie⁴^ORCID

Affiliation:

1. The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China

2. Bioinspired Engineering and Biomechanics Center (BEBC), Xi’an Jiaotong University, Xi’an 710049, China

3. Public Teaching Department, Maanshan Teacher’s College, Maanshan 243041, China

4. Suzhou Diyinan Biotech Company, Suzhou 215129, China

5. School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou 215009, China

Abstract

Exercise-induced muscle injury is one of the most common types of sports injuries. Skeletal muscle troponin I (skTnI) serves as an ideal biomarker in assessing such injuries, facilitating timely detection and evaluation. In this study, we develop a fluorescent sandwich lateral flow immunoassay (LFIA) combined with a desktop analyzer for rapid detection of skTnI. Through optimizing the reaction system, the assay achieves a satisfying detection performance, reaching a limit of detection (LOD) of 0.5 ng/mL with a turnaround time of 15 min. The proposed detection platform offers portability, ease of use, and high sensitivity, which facilitates the monitoring of exercise-induced muscle injuries at the point of care. This feature is particularly advantageous for end users, enabling timely detection of sports-related injuries and ultimately enhancing prognosis and sports life.

Funder

Natural Science Research Project for Anhui Universities of the Key Scientific Research Foundation of the Education Department of Province Anhui

Anhui Universities Quality Project of Teaching Research Key Project

Publisher

MDPI AG

Link

https://www.mdpi.com/2079-6374/14/8/381/pdf

Reference18 articles.

1. Changes in serum fast and slow skeletal troponin I concentration following maximal eccentric contractions;Chapman;J. Sci. Med. Sport,2013

2. Effect of Curcumin Supplementation on Exercise-Induced Oxidative Stress, Inflammation, Muscle Damage, and Muscle Soreness;Ms;J. Diet. Suppl.,2020

3. Diagnosis and prognosis for exercise-induced muscle injuries: From conventional imaging to emerging point-of-care testing;Tang;RSC Adv.,2020

4. Doral, M.N., and Karlsson, J. (2015). Muscle Injuries: Strains, Contusions, and Ruptures. Sports Injuries: Prevention, Diagnosis, Treatment and Rehabilitation, Springer.

5. Eccentric contractions leading to DOMS do not cause loss of desmin nor fibre necrosis in human muscle;Yu;Histochem. Cell Biol.,2002