Imaging of Lipid Peroxidation-Associated Chemiluminescence in Plants: Spectral Features, Regulation and Origin of the Signal in Leaves and Roots

Abstract

Plants, like most living organisms, spontaneously emit photons of visible light. This ultraweak endogenous chemiluminescence is linked to the oxidative metabolism, with lipid peroxidation constituting a major source of photons in plants. We imaged this signal using a very sensitive cooled CCD camera and analysed its spectral characteristics using bandpass interference filters. In vitro oxidation of lipids induced luminescence throughout the visible spectrum (450–850 nm). However, luminescence in the red spectral domain (>640 nm) occurred first, then declined in parallel with the appearance of the emission in the blue-green (<600 nm). This temporal separation suggests that the chemical species emitting in the blue-green are secondary products, possibly deriving from the red light-emitting species. This conversion did not seem to occur in planta because spontaneous chemiluminescence from plant tissues (leaves, roots) occurred only in the red/far-red light domain (>640 nm), peaking at 700–750 nm. The spectrum of plant chemiluminescence was independent of chlorophyll. The in vivo signal was modulated by cellular detoxification mechanisms and by changes in the concentration of singlet oxygen in the tissues, although the singlet oxygen luminescence bands did not appear as major bands in the spectra. Our results indicate that the intensity of endogenous chemiluminescence from plant tissues is determined by the balance between the formation of luminescent species through secondary reactions involving lipid peroxide-derived intermediates, including singlet oxygen, and their elimination by metabolizing processes. The kinetic aspects of plant chemiluminescence must be taken into account when using the signal as an oxidative stress marker.