Affiliation:
1. Institute of Pharmaceutical Science, King’s College London, London SE1 9NH, UK
2. Centre for Therapeutic Innovation, University of Bath, Bath BA2 7AY, UK
Abstract
An aromatic substrate for hydroxylation by hydroxyl radicals (•OH) was investigated. The probe, N,N’-(5-nitro-1,3-phenylene)-bis-glutaramide, and its hydroxylated product do not bind either iron(III) or iron(II), and so they do not interfere with the Fenton reaction. A spectrophotometric assay based on the hydroxylation of the substrate was developed. The synthesis and purification methods of this probe from previously published methodologies were improved upon, as well as the analytical procedure for monitoring the Fenton reaction through its use, enabling univocal and sensitive •OH detection. The assay was utilised to demonstrate that the iron(III) complexes of long-chain fatty acids lack Fenton activity under biological conditions.
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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