DNA Methylome and Transcriptome Maps of Primary Colorectal Cancer and Matched Liver Metastasis

Author:

Ajithkumar Priyadarshana1,Gimenez Gregory1,Stockwell Peter A.1ORCID,Almomani Suzan1,Bowden Sarah A.1,Leichter Anna L.1ORCID,Ahn Antonio23,Pattison Sharon4ORCID,Schmeier Sebastian5,Frizelle Frank A.6,Eccles Michael R.1ORCID,Purcell Rachel V.6,Rodger Euan J.1ORCID,Chatterjee Aniruddha17ORCID

Affiliation:

1. Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand

2. Peter MacCallum Cancer Centre, Melbourne, VIC 3052, Australia

3. The Sir Peter MacCallum Department of Oncology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Melbourne, VIC 3052, Australia

4. Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand

5. Evotec SE, 22419 Hamburg, Germany

6. Department of Surgery, University of Otago, Christchurch 8011, New Zealand

7. UPES University, Dehradun 248007, India

Abstract

Sequencing-based genome-wide DNA methylation, gene expression studies and associated data on paired colorectal cancer (CRC) primary and liver metastasis are very limited. We have profiled the DNA methylome and transcriptome of matched primary CRC and liver metastasis samples from the same patients. Genome-scale methylation and expression levels were examined using Reduced Representation Bisulfite Sequencing (RRBS) and RNA-Seq, respectively. To investigate DNA methylation and expression patterns, we generated a total of 1.01 × 109 RRBS reads and 4.38 × 108 RNA-Seq reads from the matched cancer tissues. Here, we describe in detail the sample features, experimental design, methods and bioinformatic pipeline for these epigenetic data. We demonstrate the quality of both the samples and sequence data obtained from the paired samples. The sequencing data obtained from this study will serve as a valuable resource for studying underlying mechanisms of distant metastasis and the utility of epigenetic profiles in cancer metastasis.

Funder

Royal Society of New Zealand Te Apārangi

Lottery Health Research New Zealand

University of Otago

Maurice and Phyllis Paykel Trust

Gut Cancer Foundation NZ

Urology and Department of Surgical Sciences

Hugh Green Foundation

Colorectal Surgical Society of Australia and New Zealand

Maurice Wilkins Centre for Molecular Biodiscovery

Cancer Society of New Zealand

Health Research Council of New Zealand

New Zealand Institute for Cancer Research Trust

Cancer Research Trust New Zealand

Publisher

MDPI AG

Subject

Information Systems and Management,Computer Science Applications,Information Systems

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Exploring Potential Epigenetic Biomarkers for Colorectal Cancer Metastasis;International Journal of Molecular Sciences;2024-01-10

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