Affiliation:
1. College of Agriculture and Life Sciences, Kunming University, Kunming 650214, China
2. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China
3. Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650224, China
4. Key Laboratory of Breeding and Utilization of Resource Insects of National Forestry and Grassland Administration, Kunming 650224, China
Abstract
Fenugreek (Trigonella foenum-graecum L.) is a traditional medicinal plant for treating human diseases that is widely cultivated in many countries. However, the component and related metabolic pathways are still unclear. To understand the changes in expression of the component and related genes during seed development, this study employed metabolomic and transcriptomic analyses and integrative analysis to explore the metabolites and pathways involved in the growth of fenugreek. The antifungal activity of the fenugreek seeds was also analyzed. A total of 9499 metabolites were identified in the positive ion mode, and 8043 metabolites were identified in the negative ion mode. Among them, the main components were fatty acyls, prenol lipids, steroids, steroid derivatives, flavonoids, and isoflavonoids. Among these enriched pathways, the top 20 pathways were “flavone and flavonol biosynthesis”, “isoflavonoid biosynthesis”, and “flavonoid biosynthesis”. 3,7-Di-O-methylquercetin, flavonoids, pseudobaptigenin, isoflavonoids, methylecgonine, alkaloids, and derivatives were the most significantly upregulated metabolites. There were 38,137 differentially expressed genes (DEGs) identified via transcriptomic analysis. According to the KEGG pathway enrichment analysis, 147 DEGs were significantly enriched in “flavonoid biosynthesis”. Ten DEGs of the six key enzymes were found to be involved in three pathways related to flavonoid and alkaloid synthesis in fenugreek. The antifungal activity test revealed the inhibitory effect of the ethanol extract of fenugreek seeds on Alternaria tenuissima (Kunze)Wiltshire and Magnaporthe oryzae. These findings further prove that the use of botanical pesticides in fenugreek fruit has research value.
Funder
Scientific Research Foundation of Kunming University
Joint Funds in Basic Research for Local Universities in Yunnan Province
National Natural Science Foundation of China
Reference36 articles.
1. Wang, X.-Z., Chen, Y., Wu, Y., Shan, F.-B., Du, R.-X., Yan, W.-Z., and Liu, C.-H. (2021). Fenugreek transcriptome sequencing and bioinformatics analysis. Mol. Plant Breed., 1–16.
2. Fenugreek Cultivation with Emphasis on Historical Aspects and its uses in Traditional Medicine and Modern Pharmaceutical Science;Sun;Mini Rev. Med. Chem.,2021
3. Pharmacological Attributes of Fenugreek with Special Reference to Alzheimer’s Disease;Varshney;Curr. Alzheimer Res.,2023
4. The Effects of Fenugreek Extract on Growth Performance, Serum Biochemical Indexes, Immunity and NF-κB Signaling Pathway in Broiler;Huang;Front. Vet. Sci.,2022
5. Characterization of flavonoid glycosides from fenugreek (Trigonella foenum-graecum) crude seeds by HPLC-DAD-ESI/MS analysis;Benayad;Int. J. Mol. Sci.,2014