Stomatal Responses of Two Drought-Tolerant Barley Varieties with Different ROS Regulation Strategies under Drought Conditions

Abstract

Drought stress is a major obstacle to agricultural production. Stomata are central to efforts to improve photosynthesis and water use. They are targets for manipulation to improve both processes and the balance between them. An in-depth understanding of stomatal behavior and kinetics is important for improving photosynthesis and the WUE of crops. In this study, a drought stress pot experiment was performed, and a transcriptome analysis of the leaves of three contrasting, cultivated barley genotypes Lumley (Lum, drought-tolerant), Golden Promise (GP, drought-sensitive), and Tadmor (Tad, drought-tolerant), generated by high-throughput sequencing, were compared. Lum exhibited a different WUE at the leaf and whole-plant levels and had greater CO2 assimilation, with a higher gs under drought stress. Interestingly, Lum showed a slower stomatal closure in response to a light–dark transition and significant differences compared to Tad in stomatal response to the exogenous application of ABA, H2O2, and CaCl2. A transcriptome analysis revealed that 24 ROS-related genes were indeed involved in drought response regulation, and impaired ABA-induced ROS accumulation in Lum was identified using ROS and antioxidant capacity measurements. We conclude that different stomatal ROS responses affect stomatal closure in barley, demonstrating different drought regulation strategies. These results provide valuable insight into the physiological and molecular basis of stomatal behavior and drought tolerance in barley.