Molecular and Genetic Characterization of Colicinogenic Escherichia coli Strains Active against Shiga Toxin-Producing Escherichia coli O157:H7-Reference-Cited by-同舟云学术

Molecular and Genetic Characterization of Colicinogenic Escherichia coli Strains Active against Shiga Toxin-Producing Escherichia coli O157:H7

Published:2023-07-11 Issue:14 Volume:12 Page:2676
ISSN:2304-8158
Container-title:Foods
language:en
Short-container-title:Foods

Author:

García Mauro D.¹,Ruiz María J.¹^ORCID,Medina Luis M.²^ORCID,Vidal Roberto³,Padola Nora L.¹,Etcheverria Analía I.¹

Affiliation:

1. Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, CICPBA, Facultad de Ciencias Veterinarias, UNICEN-Campus Universitario, Tandil B7000, Argentina

2. Food Science and Technology Department, Faculty of Veterinary Medicine, Universidad de Cordoba, 14071 Córdoba, Spain

3. Instituto de Ciencias biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile

Abstract

The objective of this work was to molecularly and genotypically characterize and test the inhibitory activity of six colicinogenic Escherichia coli strains (ColEc) and their partially purified colicins against STEC O157:H7 isolated from clinical human cases. Inhibition tests demonstrated the activity of these strains and their colicins against STEC O157:H7. By PCR it was possible to detect colicins Ia, E7, and B and microcins M, H47, C7, and J25. By genome sequencing of two selected ColEc strains, it was possible to identify additional colicins such as E1 and Ib. No genes coding for stx1 and stx2 were detected after analyzing the genome sequence. The inhibitory activity of ColEc against STEC O157:H7 used as an indicator showed that colicins are potent growth inhibitors of E. coli O157:H7, being a potential alternative to reduce the presence of pathogens of public health relevance.

Funder

CONICET, Argentina

University of Cordoba

Publisher

MDPI AG

Subject

Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science

Link

https://www.mdpi.com/2304-8158/12/14/2676/pdf

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