Cadmium-Tolerant Bacteria in Cacao Farms from Antioquia, Colombia: Isolation, Characterization and Potential Use to Mitigate Cadmium Contamination

Abstract

Bioremediation of farm soil is a technique that merits in-depth research. There are few studies related to the use of bioremediation to reduce cadmium (Cd) availability in soils used for cacao production. This study investigates (1) field bioprospection and strain characterization using techniques including isothermal microcalorimetry to select a group of cadmium-tolerant bacteria (CdtB) for potential use as bioremediators of cacao soils and (2) the application of bacterial inoculum to compare the immobilization of Cd under field conditions. Bioprospection was carried out in four cacao farms from the Antioquia district in Colombia. Culturable CdtB strains were isolated using CdCl2 as a Cd source and identified using molecular techniques. The metabolic characterization of Cd immobilization was carried out using isothermal microcalorimetry with CdCl2 amendments. Five cadmium-tolerant bacteria were isolated and characterized as Bacillus spp. The strain CdtB14 showed better growth and Cd immobilization ability (estimated through heat ratios) than any strain isolated thus far, suggesting potential for future use in bioproduct development. Furthermore, the application of two previously characterized CdtB strains with zeolite powder was performed in the same farms where the bioprospection process was carried out. The application of the preformulated inoculum resulted in a decrease of 0.30 + 0.1 mg kg−1 of soil Cd in two out of the four assessed farms. The field results are preliminary and require data on the change in Cd in cacao beans to understand what this result means for Cd mitigation. This study is the first to combine bioprospecting and the performance of CdtB in laboratory and field experiments in cacao farms and shows the potential of bioremediation to mitigate Cd contamination in cacao.