Evaluation of Behavioral Changes and Tissue Damages in Common Carp (Cyprinus carpio) after Exposure to the Herbicide Glyphosate

Abstract

Pesticides can induce changes in behavior and reduce the survival chance of aquatic organisms. In this study, the toxic effects of glyphosate suspension (Glyphosate Aria 41% SL, Tehran Iran) on behavior and tissues of common carp (Cyprinus carpio) were assessed. For this purpose, a 96 h LC50 of glyphosate suspension (68.788 mL·L−1) was used in the toxicity test. All individuals were divided into control and treatment groups with four replicates. Exposure operations were performed under two conditions: increasing concentration of suspension from 0 to 68.788 mL·L−1; then, decreasing to the first level. The swimming pattern was recorded by digital cameras during the test and tissue samples were collected at the end of the test. There were significant differences between the swimming pattern of treated individuals and control ones during both steps. The sublethal concentration of glyphosate led to hypertrophy, hyperplasia and hyperemia in the gill of fish. However, changes were obvious only after sampling. The exposed fish also displayed clinical signs such as darkening of the skin and increasing movement of the operculum. Moreover, glyphosate suspension affected swimming patterns of fish suggest that the swimming behavior test can indicate the potential toxicity of environmental pollutants and be used as a noninvasive, useful method for managing environmental changes and assessing fish health conditions by video monitoring.