Abstract
Gelsemium elegans (G. elegans) is a Chinese medicinal plant with substantial economic and feeding values. There is a lack of detailed studies on the mitochondrial genome of G. elegans. In this study, the mitochondrial genome of G. elegans was sequenced and assembled, and its substructure was investigated. The mitochondrial genome of G. elegans is represented by two circular chromosomes of 406,009 bp in length with 33 annotated protein-coding genes, 15 tRNA genes, and three rRNA genes. We detected 145 pairs of repeats and found that four pairs of repeats could mediate the homologous recombination into one major conformation and five minor conformations, and the presence of conformations was verified by PCR amplification and Sanger sequencing. A total of 124 SSRs were identified in the G. elegans mitochondrial genome. The homologous segments between the chloroplast and mitochondrial genomes accounted for 5.85% of the mitochondrial genome. We also predicted 477 RNA potential editing sites and found that the nad4 gene was edited 38 times, which was the most frequent occurrence. Taken together, the mitochondrial genome of G. elegans was assembled and annotated. We gained a more comprehensive understanding on the genome of this medicinal plant, which is vital for its effective utilization and genetic improvement, especially for cytoplasmic male sterility breeding and evolution analysis in G. elegans.
Funder
the Youth Project of Health Department of Fujian Province
the Innovation Joint Project of Fujian Science and Technology
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference67 articles.
1. Whole-genome sequencing and analysis of the Chinese herbal plant Gelsemium elegans;Liu;Acta Pharm. Sin. B,2020
2. Lin, H.L., Qiu, H.Q., Cheng, Y., Liu, M., Chen, M.H., Que, Y.X., and Que, W.C. (2021). Gelsemium elegans Benth: Chemical components, pharmacological effects, and toxicity mechanisms. Molecules, 26.
3. Research progress on chemical constituents and pharmacological effects of Gelsemium elegans Benth;Wang;J. Tradit. Chin. Med.,2016
4. Wu, J.W. (2015). Study on the Promotion of Growth in Pigs by Gelsemiumelegans (Gardncechamp) Benth. [Ph.D. Thesis, Fujian Agriculture and Forestry University].
5. Antineoplastic effect of koumine in mice bearing H22 solid tumor;Cai;J. South. Med. Univ.,2009
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