Diversity, Phylogenetic Relationships and Distribution of Marsh Frogs (the Pelophylax ridibundus complex) from Kazakhstan and Northwest China

Abstract

In order to study the diversity, phylogenetic relationships and distribution of marsh frogs of the Pelophylax ridibundus complex in Kazakhstan and northwest China, we conducted phylogeographic analyses of 125 samples from 53 localities using the mtDNA ND2 and COI genes and the SAI nuclear gene sequences. Phylogenetic inference of mtDNA revealed three main lineages—sister lineages Balkhash and Syrdarya (as the Central Asian P. sp. novum), and the Anatolian P. cf. bedriagae, while from nDNA data, we additionally detected the western form, P. ridibundus. According to mtDNA data, the mean genetic distances between P. sp. novum and two other forms of marsh frogs was more than 5%. Genetic homogeneity within populations of the Syrdarya lineage and P. cf. bedriagae is characterized by low nucleotide diversity and high haplotype diversity. Demographic analyses of the lineages showed past population expansions of the Balkhash and the Syrdarya forms. Divergence from the most recent ancestor had occurred in the Early Pleistocene period (2.46 Mya) for the Balkhash and the Syrdarya lineages, and 1.27 Mya for the P. cf. bedriagae. Our findings provide a first investigation of the lineage diversification and population dynamics of the Central Asian marsh frogs and will be useful for further taxonomic implications and conservational actions.