Transcriptome Reveals the Key Genes Related to the Metabolism of Volatile Sulfur-Containing Compounds in Lentinula edodes Mycelium

Author:

Li Zheng1,Pan Fei2ORCID,Huang Wen1ORCID,Gao Shuangshuang1,Feng Xi3,Chang Meijie1,Chen Lianfu4,Bian Yinbing4,Tian Wenli2,Liu Ying1ORCID

Affiliation:

1. College of Food Science & Technology, Huazhong Agricultural University, Wuhan 430070, China

2. State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, China

3. Department of Nutrition, Food Science and Packaging, San Jose State University, San Jose, CA 95192, USA

4. Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070, China

Abstract

Lentinula edodes (L. edodes) is a globally popular edible mushroom because of its characteristic sulfur-containing flavor compounds. However, the formation of the volatile sulfur-containing compounds in the mycelium of L. edodes has not been studied. We found that there were also sulfur-containing aroma compounds in the mycelium of L. edodes, and the content and composition varied at different stages of mycelial growth and development. The γ-glutamyl-transpeptidase (GGT) and cysteine sulfoxide lyase (C-S lyase) related to the generation of sulfur compounds showed the highest activities in the 15-day sample. Candidate genes for the metabolism of volatile sulfur compounds in mycelium were screened using transcriptome analysis, including encoding the GGT enzyme, C-S lyase, fatty acid oxidase, HSP20, and P450 genes. The expression patterns of Leggt3 and Leccsl3 genes were consistent with the measured activities of GGT and C-S lyase during the cultivation of mycelium and molecular dynamics simulations showed that they could stably bind to the substrate. Our findings provide insights into the formation of sulfur-containing flavor compounds in L. edodes. The mycelium of L. edodes is suggested for use as material for the production of sulfur-containing flavor compounds.

Funder

Natural Science Foundation of China

Key Research and Development Projects in Agricultural and Rural Areas of Hubei Province

Agricultural Science and Technology Innovation Center of Hubei Province

Hubei Agriculture Research System

Publisher

MDPI AG

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