A Genomic Sequence Resource of Diaporthe mahothocarpus GZU-Y2 Causing Leaf Spot Blight in Camellia oleifera

Abstract

Diaporthe mahothocarpus GZU-Y2, a new pathogen responsible for leaf spot blight disease, leads to significant damage and economic losses in some Camellia oleifera plantations. The current study annotated the genome of the D. mahothocarpus isolate GZU-Y2 to advance our knowledge of the pathogen and facilitate improved disease management of leaf spot blight. The initial Pacbio-Illumina hybrid draft genome for GZU-Y2 resulted in a high-quality assembly with 62 contigs, characterized by an N50 length of 7.07 Mb. The complete genome of isolate GZU-Y2 was 58.97 Mbp, with a GC content of 50.65%. Importantly, the assembly exhibits remarkable integrity, with 97.93% of complete BUSCO validating genome completeness. The prediction results showed that a total of 15,918 protein-coding genes were annotated using multiple bioinformatics databases. The genome assembly and annotation resource reported here will be useful for the further study of fungal infection mechanisms and pathogen–host interaction.