Affiliation:
1. Center for Global Health, The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China
2. Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, New Valley University, New Valley 72713, Egypt
Abstract
The molecular mechanism of the hepatotoxicant aflatoxin B1 to induce liver fibrosis and hepatocellular carcinoma (HCC) remains unclear, to offer fresh perspectives on the molecular mechanisms underlying the onset and progression of AFB1-Fibrosis-HCC, which may offer novel targets for the detection and therapy of HCC caused by AFB1. In this study, expression profiles of AFB1, liver fibrosis and liver cancer-related datasets were downloaded from the Gene Expression Omnibus (GEO), and differentially expressed genes (DEGs) were identified by the GEO2R tool. The STRING database, CytoHubba, and Cytoscape software were used to create the protein-protein interaction and hub genes of the combined genes, and the ssGSEA score for inflammatory cells related gene sets, the signaling pathway, and immunotherapy were identified using R software and the GSEA database. The findings revealed that AFB1-associated liver fibrosis and HCC combined genes were linked to cell process disruptions, the BUB1B and RRM2 genes were identified as hub genes, and the BUB1B gene was significantly increased in JAK-STAT signaling gene sets pathways as well as having an immunotherapy-related impact. In conclusion, BUB1B and RRM2 were identified as potential biomarkers for AFB1-induced fibrosis and HCC progression.
Funder
Natural Science Foundations of China
Priority Academic Program Development of Jiangsu Higher Education Institutions
Subject
General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology
Reference39 articles.
1. Liver cirrhosis;Tsochatzis;Lancet,2014
2. Cancer statistics;Siegel;Cancer J. Clin.,2016
3. Does aflatoxin exposure in the United Kingdom constitute a cancer risk?;Harrison;Environ. Health Perspect.,1993
4. DNA damage by mycotoxins;Wang;Mutat. Res. Fundam. Mol. Mech. Mutagen.,1999
5. Dietary aflatoxin B1 intake, genetic polymorphisms of CYP1A2, CYP2E1, EPHX1, GSTM1, and GSTT1, and gastric cancer risk in Korean;Eom;Cancer Causes Control.,2013
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