Cloning and Functional Analysis of CsROP5 and CsROP10 Genes Involved in Cucumber Resistance to Corynespora cassiicola

Author:

Yu Guangchao12,Jia Lian12,Yu Ning12,Feng Miao12,Qu Yue12

Affiliation:

1. College of Chemistry and Life Sciences, Anshan Normal University, Anshan 114007, China

2. Liaoning Key Laboratory of Development and Utilization for Natural Products Active Molecules, Anshan Normal University, Anshan 114007, China

Abstract

The cloning of resistance-related genes CsROP5/CsROP10 and the analysis of their mechanism of action provide a theoretical basis for the development of molecular breeding of disease-resistant cucumbers. The structure domains of two Rho-related guanosine triphosphatases from plant (ROP) genes were systematically analyzed using the bioinformatics method in cucumber plants, and the genes CsROP5 (Cucsa.322750) and CsROP10 (Cucsa.197080) were cloned. The functions of the two genes were analyzed using reverse-transcription quantitative PCR (RT-qPCR), virus-induced gene silencing (VIGS), transient overexpression, cucumber genetic transformation, and histochemical staining technology. The conserved elements of the CsROP5/CsROP10 proteins include five sequence motifs (G1-G5), a recognition site for serine/threonine kinases, and a hypervariable region (HVR). The knockdown of CsROP10 through VIGS affected the transcript levels of ABA-signaling-pathway-related genes (CsPYL, CsPP2Cs, CsSnRK2s, and CsABI5), ROS-signaling-pathway-related genes (CsRBOHD and CsRBOHF), and defense-related genes (CsPR2 and CsPR3), thereby improving cucumber resistance to Corynespora cassiicola. Meanwhile, inhibiting the expression of CsROP5 regulated the expression levels of ROS-signaling-pathway-related genes (CsRBOHD and CsRBOHF) and defense-related genes (CsPR2 and CsPR3), thereby enhancing the resistance of cucumber to C. cassiicola. Overall, CsROP5 and CsROP10 may participate in cucumber resistance to C. cassiicola through the ROS and ABA signaling pathways.

Funder

Scientific Research Project of Liaoning Education Department

Publisher

MDPI AG

Reference77 articles.

1. Fine genetic mapping of target leaf spot resistance gene cca3 in cucumber, Cucumis sativus L.;Wen;Theor. Appl. Genet.,2015

2. First characterization of endophytic Corynespora cassiicola isolates with variant cassicolin genes recovered from rubber trees in Brazil;Scomparin;Fungal Divers.,2012

3. Characterization of a cassicolin-encoding gene from Corynespora cassiicola, pathogen of rubbertree (Hevea brasiliensis);Gimenez;Plant Sci.,2012

4. Diversity of the Cassicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis;Fumanal;Fungal Biol.,2014

5. Analysis of pathogenic and genetic variability of Corynespora cassicola based on iPBSretrotransposons;Wu;Can. J. Plant Pathol.,2018

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3