Glucose Oxidase Captured into Electropolymerized p-Coumaric Acid towards the Development of a Glucose Biosensor

Abstract

An electrochemical biosensor based on the immobilization of glucose oxidase into an electropolymerized p-coumaric acid membrane on a Pt electrode has been developed and evaluated for glucose detection in the range of 1 to 30 mM. The glucose biosensor exhibits a sensitivity of 36.96 mA/mMcm2, a LOD of 0.66 mM, and a LOQ of 2.18 mM. The biosensing membrane was electropolymerized by cyclic voltammetry in 100 mM phosphates pH 7.00 and 3% ethanol containing glucose oxidase and p-coumaric acid. The glucose biosensors’ stability, repeatability, reproducibility, and selectivity were estimated. The biosensing membrane shows permselective properties and antifouling effects. The applicability of the developed glucose biosensor was evaluated in the presence of 20 mg/mL proteins, and any signal associated with biofouling was observed. The glucose biosensors were employed for the determination of the glucose concentration in three commercial beverages.